Rp-hplc Method for Simultaneous Estimation of Losartan, Hydrochlorothiazide and Amlodipine in Tablet Dosage Form

Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Losartan, Hydrochlorothiazide and Amlodipine from bulk and formulations. Methods: Reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for rapid analysis of simultaneous determination of Losartan, Hydrochlorothiazide and Amlodipine. The separation of these three drugs was achieved on a Hypersil Gold column (250 mm X 4.6 mm, 5 μ) as stationary phase with a mobile phase consisting of methanol: water in the ratio of 95:5% v/v at a flow rate of 0.8 mL/min and UV detection at 230 nm. Results: The retention times were observed to be 2.850, 3.875 and 7.333 minutes for Losartan, Hydrochlorothiazide and Amlodipine, respectively. The method was statistically validated for linearity, recovery, limit of detection, limit of quantification, accuracy and precision. Conclusion: The method was successfully applied for analysis of combined dose tablet formulation containing Losartan, Hydrochlorothiazide and Amlodipine.